Adding a new dimension to DNA melting curvesS. Cuesta-Lopez1, D. Angelov2 and M. Peyrard1
1 Université de Lyon, Ecole Normale Supérieure de Lyon, Laboratoire de Physique CNRS UMR 5672 46 allée d'Italie, 69364 Lyon Cedex 07, France, EU
2 Université de Lyon, Ecole Normale Supérieure de Lyon, Laboratoire de Biologie Moléculaire de la Cellule CNRS UMR 5239 - 46 allée d'Italie, 69364 Lyon Cedex 7, France, EU
received 12 May 2009; accepted in final form 10 August 2009; published August 2009
published online 8 September 2009
Standard DNA melting curves record the separation of the two strands vs. temperature, but they do not provide any information on the location of the opening. We introduce an experimental method which adds a new dimension to the melting curves of short DNA sequences by allowing us to record the degree of opening in several positions along the molecule all at once. This adds the spatial dimension to the melting curves and allows a precise investigation of the role of the base pair sequence on the fluctuations and denaturation of the DNA double helix. We illustrate the power of the method by investigating the influence of an AT-rich region on the fluctuations of neighboring domains.
87.15.hp - Conformational changes.
82.39.Pj - Nucleic acids, DNA and RNA bases.
87.15.Cc - Folding: thermodynamics, statistical mechanics, models, and pathways.
© EPLA 2009