Tension-induced pore formation and leakage in adhering vesiclesP. Lenz1, J. M. Johnson2, 3, Y.-H. M. Chan2 and S. G. Boxer2
1 Philipps-Universität Marburg, Fachbereich Physik - Renthof 5 D-35032 Marburg, Germany
2 Department of Chemistry, Stanford University - Stanford, CA 94305, USA
3 Department of Physiology and Biophysics, University of Colorado Health Sciences Center - Denver, CO 80045, USA
received 18 February 2006; accepted in final form 27 June 2006
published online 14 July 2006
The influence of inclusion-induced tension on pore formation is studied theoretically and experimentally. It is shown that fluorescently labeled lipids can enhance pore formation and induce leakage of adhering vesicles. These effects are more pronounced for smaller vesicles. The theoretical predictions are confirmed by experimental two-color fluorescent data. Finally, the influence of the pore formation dynamics on rupture processes of vesicles is analyzed yielding a new picture of the transition to bilayer disks.
87.16.Dg - Membranes, bilayers, and vesicles.
68.35.Np - Adhesion.
© EDP Sciences 2006