Issue
EPL
Volume 81, Number 5, March 2008
Article Number 56003
Number of page(s) 6
Section Condensed Matter: Structural, Mechanical and Thermal Properties
DOI http://dx.doi.org/10.1209/0295-5075/81/56003
Published online 18 February 2008
EPL, 81 (2008) 56003
DOI: 10.1209/0295-5075/81/56003

Protein adsorption kinetics in different surface potentials

A. Quinn1, H. Mantz1, K. Jacobs1, M. Bellion2 and L. Santen2

1  Department of Experimental Physics, Saarland University - 66041 Saarbrücken, Germany
2  Department of Theoretical Physics, Saarland University - 66041 Saarbrücken, Germany


received 15 May 2007; accepted in final form 11 January 2008; published March 2008
published online 18 February 2008

Abstract
We have studied the adsorption kinetics of the protein amylase at solid/liquid interfaces. Offering substrates with tailored properties, we are able to separate the impact of short- and long-range interactions. By means of a colloidal Monte Carlo approach including conformational changes of the adsorbed proteins induced by density fluctuations, we develop a scenario that is consistent with the experimentally observed three-step kinetics on specific substrates. Our observations show that not only the surface chemistry determines the properties of an adsorbed protein layer but also the van der Waals contributions of a composite substrate may lead to non-negligible effects.

PACS
68.47.Pe - Langmuir-Blodgett films on solids; polymers on surfaces; biological molecules on surfaces.
68.43.-h - Chemisorption/physisorption: adsorbates on surfaces.
87.14.E- - Proteins.

© EPLA 2008