Protein adsorption kinetics in different surface potentialsA. Quinn1, H. Mantz1, K. Jacobs1, M. Bellion2 and L. Santen2
1 Department of Experimental Physics, Saarland University - 66041 Saarbrücken, Germany
2 Department of Theoretical Physics, Saarland University - 66041 Saarbrücken, Germany
received 15 May 2007; accepted in final form 11 January 2008; published March 2008
published online 18 February 2008
We have studied the adsorption kinetics of the protein amylase at solid/liquid interfaces. Offering substrates with tailored properties, we are able to separate the impact of short- and long-range interactions. By means of a colloidal Monte Carlo approach including conformational changes of the adsorbed proteins induced by density fluctuations, we develop a scenario that is consistent with the experimentally observed three-step kinetics on specific substrates. Our observations show that not only the surface chemistry determines the properties of an adsorbed protein layer but also the van der Waals contributions of a composite substrate may lead to non-negligible effects.
68.47.Pe - Langmuir-Blodgett films on solids; polymers on surfaces; biological molecules on surfaces.
68.43.-h - Chemisorption/physisorption: adsorbates on surfaces.
87.14.E- - Proteins.
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