Detection of DNA hybridisation on a functionalised diamond surface using reflection anisotropy spectroscopyC. I. Smith1, A. Bowfield1, M. C. Cuquerella1, C. P. Mansley1, T. Farrell1, P. Harrison1, D. S. Martin1, D. G. Fernig2, C. Edwards2, J. E. Butler3, R. J. Hamers4, B. Sun4, X. Wang4 and P. Weightman1
1 Department of Physics, University of Liverpool - Liverpool, L69 7ZE, UK, EU
2 School of Biological Sciences, University of Liverpool - Liverpool, L69 7ZB, UK, EU
3 Code 6174, Naval Research Laboratory - Washington, DC 20375, USA
4 Chemistry Department, University of Wisconsin-Madison - 1101 University Avenue Madison, WI 53706 USA
received 23 October 2008; accepted in final form 28 November 2008; published January 2009
published online 7 January 2009
The analysis of single-stranded DNA attached to a polycrystalline diamond surface by reflection anisotropy spectroscopy (RAS) demonstrates that the DNA is oriented essentially vertically to the surface. RAS is able to detect the hybridisation between the attached strand and the homologous sequence.
87.80.Dj - Spectroscopies.
81.05.Uw - Carbon, diamond, graphite.
87.14.gk - DNA.
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