Twist-bend instability for toroidal DNA condensates

Max-Planck-Institut für Polymerforschung - Ackermannweg 10, 55128 Mainz, Germany

Received: 17 March 2004
Accepted: 19 May 2004

Abstract

We propose that semiflexible polymers in poor solvent collapse in two stages. The first stage is the well-known formation of a dense toroidal aggregate. However, if the solvent is sufficiently poor, the condensate will undergo a second structural transition to a twisted entangled state, in which individual filaments lower their bending energy by additionally orbiting around the mean path along which they wind. This “topological ripening” is consistent with known simulations and experimental results. It connects and rationalizes various experimental observations ranging from strong DNA entanglement in viral capsids to the unusually short pitch of the cholesteric phase of DNA in sperm heads. We propose that topological ripening of DNA toroids could improve the efficiency and stability of gene delivery.