| Issue |
Europhys. Lett.
Volume 69, Number 6, March 2005
|
|
|---|---|---|
| Page(s) | 1024 - 1030 | |
| Section | Interdisciplinary physics and related areas of science and technology | |
| DOI | https://doi.org/10.1209/epl/i2004-10444-6 | |
| Published online | 11 February 2005 | |
Phase transition in reconstituted chromatin
1
Department of Physics, Graduate School of Science, Kyoto University & CREST Kyoto 606-8502, Japan
2
Graduate School of Biostudies, Kyoto University - Kyoto 606-8502, Japan
Received:
23
August
2004
Accepted:
20
January
2005
By observing reconstituted chromatin by fluorescence microscopy (FM) and atomic force microscopy (AFM), we found that the density of nucleosomes exhibits a bimodal profile, i.e., there is a large transition between the dense and dispersed states in reconstituted chromatin. Based on an analysis of the spatial distribution of nucleosome cores, we deduced an effective thermodynamic potential as a function of the nucleosome-nucleosome distance. This enabled us to interpret the folding transition of reconstituted chromatin in terms of a first-order phase transition. This mechanism for the condensation of chromatin is discussed in terms of its biological significance.
PACS: 87.15.He – Dynamics and conformational changes / 64.75.+g – Solubility, segregation, and mixing; phase separation / 36.20.Ey – Conformation (statistics and dynamics)
© EDP Sciences, 2005
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