Issue |
EPL
Volume 82, Number 5, June 2008
|
|
---|---|---|
Article Number | 58001 | |
Number of page(s) | 5 | |
Section | Interdisciplinary Physics and Related Areas of Science and Technology | |
DOI | https://doi.org/10.1209/0295-5075/82/58001 | |
Published online | 23 May 2008 |
Tannin-assisted aggregation of natively unfolded proteins
1
Laboratoire de Physique Théorique et Hautes Energies - 4 Place Jussieu, BP 126, F-75252 Paris Cédex 05, France, EU
2
European Synchrotron radiation Facility - 6 rue Jules Horowitz, BP 220, F-38043 Grenoble, France, EU
3
UR117 Cidricoles et Biotransformation des Fruits et Légumes, INRA - F-35650 Le Rheu, France, EU
4
Laboratoire de Physique et Mécanique des Milieux Hétérogènes, École Supérieure de Physique et de Chimie Industrielles de la Ville de Paris - 10 rue Vauquelin, F-75231 Paris Cédex 05 - France, EU
5
INRA, Agrocampus, UMR1253 STLO - 65 rue de Saint Breuc, F-35000 Rennes, France, EU
Corresponding author: drazen@lpthe.jussieu.fr
Received:
10
March
2008
Accepted:
15
April
2008
Tannin-protein interactions are essentially physical: hydrophobic and hydrogen-bondmediated. We explored the tannin-assisted protein aggregation on the case of
-casein, which is a natively unfolded protein known for its ability to form micellar aggregates. We used several tannins with specified length. Our SAXS results show that small tannins increase the number of proteins per micelle, but keeping their size constant. It leads to a tannin-assisted compactization of micelles. Larger tannins, with linear dimensions greater than the crown width of micelles, lead to the aggregation of micelles by a bridging effect. Experimental results can be understood within a model where tannins are treated as effective enhancers of hydrophobic attraction between specific sites in proteins.
PACS: 87.15.-v – Biomolecules: structure and physical properties / 82.70.Dd – Colloids
© EPLA, 2008
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.