Volume 87, Number 4, August 2009
|Number of page(s)||6|
|Section||Interdisciplinary Physics and Related Areas of Science and Technology|
|Published online||08 September 2009|
Adding a new dimension to DNA melting curves
Université de Lyon, Ecole Normale Supérieure de Lyon, Laboratoire de Physique CNRS UMR 5672 46 allée d'Italie, 69364 Lyon Cedex 07, France, EU
2 Université de Lyon, Ecole Normale Supérieure de Lyon, Laboratoire de Biologie Moléculaire de la Cellule CNRS UMR 5239 - 46 allée d'Italie, 69364 Lyon Cedex 7, France, EU
Corresponding author: Santiago.Cuesta.Lopez@ens-lyon.fr
Accepted: 10 August 2009
Standard DNA melting curves record the separation of the two strands vs. temperature, but they do not provide any information on the location of the opening. We introduce an experimental method which adds a new dimension to the melting curves of short DNA sequences by allowing us to record the degree of opening in several positions along the molecule all at once. This adds the spatial dimension to the melting curves and allows a precise investigation of the role of the base pair sequence on the fluctuations and denaturation of the DNA double helix. We illustrate the power of the method by investigating the influence of an AT-rich region on the fluctuations of neighboring domains.
PACS: 87.15.hp – Conformational changes / 82.39.Pj – Nucleic acids, DNA and RNA bases / 87.15.Cc – Folding: thermodynamics, statistical mechanics, models, and pathways
© EPLA, 2009
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