Volume 125, Number 2, January 2019
|Number of page(s)||6|
|Published online||14 February 2019|
Spatio-temporal correlation super-resolution optical fluctuation imaging
1 Institute for Physical Chemistry, RWTH Aachen University - Landoltweg 2, 52074 Aachen, Germany
2 Department of Chemistry, KU Leuven - Celestijnenlaan 200G, 3001 Heverlee, Belgium
3 III. Institute of Physics - Biophysics, Georg August University - 37077 Göttingen, Germany
Received: 2 October 2018
Accepted: 14 January 2019
We describe a new concept of multimodal super-resolution imaging which combines the cumulant analysis from Super-resolution Optical Fluctuation Imaging (SOFI) with the imprinting of three-dimensional, spectral or other information into peculiar Point-Spread Function (PSF) patterns. This concept allows for encoding multidimensional or multimodal information into a single image plane and to extract this information by an appropriate spatio-temporal correlation analysis of emitter fluctuations. Here, we develop the general theory of this concept, and present proof-of-principle experiments of three-dimensional super-resolution imaging.
PACS: 07.60.Pb – Conventional optical microscopes / 42.30.Va – Image forming and processing / 87.64.M- – Optical microscopy
© EPLA, 2019
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