Issue |
EPL
Volume 148, Number 1, October 2024
|
|
---|---|---|
Article Number | 17002 | |
Number of page(s) | 5 | |
Section | Biological and soft matter physics | |
DOI | https://doi.org/10.1209/0295-5075/ad7dad | |
Published online | 04 October 2024 |
Dynamical stiffening of dsDNA confined and stretched in a nanochannel
1 Department of Physics, National University of Singapore - Singapore 117542, Singapore
2 Department of Physics, Indian Institute of Technology - Bhubaneswar, 752050 Odisha, India
Received: 23 August 2024
Accepted: 20 September 2024
The internal dynamics of double-stranded DNA (dsDNA) confined within channels of diameters ranging from the persistence length to twice that length were investigated using fluorescence microscopy. By analysing spatiotemporal intensity fluctuations, we derived the intermediate dynamic structure factor. The structure factor consistently exhibited behaviour characteristic of Rouse dynamics, regardless of the channel diameter. Notably, as the channel diameter decreased, the DNA molecules became increasingly elongated along the channel, leading to shorter relaxation times. These findings indicate a dynamic stiffening effect, where the effective spring constant of a stretched polymer chain increases. We propose that this effect has significant implications for controlling DNA motion in biological and biotechnological applications.
© 2024 EPLA
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