| Issue |
EPL
Volume 150, Number 5, June 2025
|
|
|---|---|---|
| Article Number | 54002 | |
| Number of page(s) | 7 | |
| Section | Nuclear and plasma physics, particles and fields | |
| DOI | https://doi.org/10.1209/0295-5075/add8b9 | |
| Published online | 09 June 2025 | |
Selective anticancer effects of plasma-activated PBS on agarose-constructed 3D tumor spheroids
School of Life and Environmental Sciences, Guilin University of Electronic Technology - Guilin 541004, China
Received: 16 November 2024
Accepted: 14 May 2025
Current research on cold atmospheric plasma-activated solutions in cancer therapy has focused on the application of plasma-activated media, neglecting the storage function of plasma-activated phosphate buffers. In this paper, we explored the optimal conditions for the construction of in vitro 3D models with low melting point agarose and investigated the selective anticancer ability of plasma-activated phosphate buffer saline (pPBS) as assessed by the concentrations of H2O2, 
and 
in pPBS. 3D cell spheres were treated using different pPBS incubation times and pPBS ratios, followed by incubation of the cell spheres. The experiments showed that cell cultures with a plasma treatment time of 7 min and 60% (v/v) pPBS had the best selective anticancer ability. We found that pPBS has a stronger ability to preserve active substances than plasma-treated medium (PAM) and is more suitable for clinical medicine.
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