Volume 67, Number 4, August 2004
|Page(s)||669 - 675|
|Section||Interdisciplinary physics and related areas of science and technology|
|Published online||01 August 2004|
Evidence of an inverted hexagonal phase in self-assembled phospholipid--metal complexes
Dipartimento di Fisica e Ingegneria dei Materiali e del Territorio and INFM Università Politecnica delle Marche - Via Brecce Bianche, 60131 Ancona, Italy
2 Dipartimento di Scienze dei Materiali e della Terra, Università Politecnica delle Marche - Via Brecce Bianche, 60131 Ancona, Italy
3 European Synchrotron Radiation Facility - BP 220, F-38043 Grenoble Cedex, France
Accepted: 7 June 2004
We report the first observation of an inverted hexagonal phase of phospholipid-DNA-metal complexes. These ternary complexes are formed in a self-assembled manner when water solutions of neutral lipid dioleoylphosphatidylethanolamine (DOPE), DNA and divalent metal cations (; , , , ) are mixed, which represents a striking example of supramolecular chemistry. The structure, derived from synchrotron X-ray diffraction, consists of cylindrical DNA strands coated by neutral lipid monolayers and arranged on a two-dimensional hexagonal lattice (). Besides the fundamental aspects, DOPE-DNA- complexes may be of great interest as efficient nonviral delivery systems in gene therapy applications because of the low inherent cytotoxicity and the potential high transfection efficiency.
PACS: 87.14.Cc – Lipids / 87.14.Gg – DNA, RNA / 61.10.-i – X-ray diffraction and scattering
© EDP Sciences, 2004
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