Volume 75, Number 4, August 2006
|Page(s)||666 - 672|
|Section||Interdisciplinary physics and related areas of science and technology|
|Published online||07 July 2006|
Tube extrusion from permeabilized giant vesicles
Laboratoire PCC Institut Curie/CNRS UMR 168 - 11 rue P. & M. Curie 75231 Paris Cedex 05, France
Corresponding author: firstname.lastname@example.org
Accepted: 20 June 2006
This letter reports the permeabilization effects of chemical additives on mechanical properties of Giant Unilamellar Vesicles (GUVs). We use a surfactant, Tween 20, inducing transient pores and a protein, Streptolysin O, inducing permanent pores in the membrane. Lipid tubes are extracted from GUVs anchored onto the tip of a micro-needle and submitted to hydrodynamic flows. On bare vesicles, tube extrusion is governed by the entropic elasticity of the membrane. The vesicle tension increases until it balances the flow velocity U and the tube reaches a stationary length. In permeabilized vesicles, the membrane tension is maintained at a constant value by the permeation of inner solution through nanometric pores. This allows extrusion of “infinite” tubes at constant velocity that never reach a stationary length. Tween-20 preliminary results suggest that strongly depends on surfactant concentration. For Streptolysin O, we have measured vs. U and found two regimes: a “high-porosity” regime for and a “low-porosity” regime for , where is related to the number of pores on the vesicle surface.
PACS: 87.16.Dg – Membranes, bilayers and vesicles / 87.15.Kg – Molecular interactions; membrane-protein interactions / 87.83.+a – Biomedical applications of nanotechnology
© EDP Sciences, 2006
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.