Volume 75, Number 4, August 2006
|Page(s)||666 - 672|
|Section||Interdisciplinary physics and related areas of science and technology|
|Published online||07 July 2006|
Tube extrusion from permeabilized giant vesicles
Laboratoire PCC Institut Curie/CNRS UMR 168 - 11 rue P. & M. Curie 75231 Paris Cedex 05, France
Corresponding author: email@example.com
Accepted: 20 June 2006
This letter reports the permeabilization effects of chemical additives on mechanical properties of Giant Unilamellar Vesicles (GUVs). We use a surfactant, Tween 20, inducing transient pores and a protein, Streptolysin O, inducing permanent pores in the membrane. Lipid tubes are extracted from GUVs anchored onto the tip of a micro-needle and submitted to hydrodynamic flows. On bare vesicles, tube extrusion is governed by the entropic elasticity of the membrane. The vesicle tension increases until it balances the flow velocity U and the tube reaches a stationary length. In permeabilized vesicles, the membrane tension is maintained at a constant value by the permeation of inner solution through nanometric pores. This allows extrusion of “infinite” tubes at constant velocity that never reach a stationary length. Tween-20 preliminary results suggest that strongly depends on surfactant concentration. For Streptolysin O, we have measured vs. U and found two regimes: a “high-porosity” regime for and a “low-porosity” regime for , where is related to the number of pores on the vesicle surface.
PACS: 87.16.Dg – Membranes, bilayers and vesicles / 87.15.Kg – Molecular interactions; membrane-protein interactions / 87.83.+a – Biomedical applications of nanotechnology
© EDP Sciences, 2006
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